|
KMID : 1005220160310030293
|
|
Journal of Embryo Transfer
2016 Volume.31 No. 3 p.293 ~ p.297
|
|
|
Association study analysis of phospholipase C zeta gene polymorphism forsperm motility and kinematic characteristics in liquid semen of Boar
|
|
Jeong Yong-Dae
Jeong Jin-Young
Sa Soo-Jin
Kim Ki-Hyun
Cho Eun-Seok
Yu Dong-Jo
Park Sung-Kwon
Jang Hyun-Jun
Woo Jae-Seok
Choi Jung-Woo
|
|
|
Abstract
|
|
|
|
For evaluating the boar semen quality, sperm motility is an important parameter because the movement of sperm indicates active metabolism, membrane integrity and fertilizing capacity. Phospholipase C zeta (PLCz) is important enzyme in spermatogenesis, but the effect has not been confirmed in pigs yet. Therefore, this study was aimed to analyze their association with sperm motility and kinematic characteristics. DNA samples from 124 Duroc pigs with records of sperm motility and kinematic characteristics [total motile spermatozoa (MOT), curvilinear velocity (VCL), straight-line velocity (VSL), the ratio between VSL and VCL (LIN), amplitude of lateral head displacement (ALH)] were subjected. A SNP in non-coding region of PLCz g.158 A > C was associated with MOT (p < 0.05), VCL (p < 0.01), LIN (p < 0.01) and ALH (p < 0.05) in Duroc population. Therefore, we suggest that the intron region of the porcine PLCz gene may be used as a molecular marker for Duroc boar semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.
|
|
|
KEYWORD
|
|
|
Phospholipase C zeta , Boar semen motility and kinematic characteristics , Polymorphism
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|